CHE301 Assignment 2 Solution 2021

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CHE301 ASSIGNMENT 2 SOLUTION SPRING 2021

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Question 1:

What are the Pros and Cons of High-Performance Liquid Chromatography?

Solution:

Pros:

HPLC offers a rapid, automated, and highly precise method to recognize certain chemical components in a sample.
High-performance liquid chromatography offers a fast and precise quantitative analysis.
A gradient solvent system can be applied in certain methods.
It is highly reproducible.
HPLC can be upgraded to mass spectroscopy.
The HPLC is very rapid, efficient, and delivers high resolution as compared to other chromatographic techniques, such as TLC, column chromatography, and paper chromatography.

Cons:

HPLC can be an expensive method, it required a large number of expensive organics, needs a power supply, and regular maintenance is required.
It can be complicated to troubleshoot problems or develop new methods.
The lack of a universal detector for HPLC, however, the UV-Vis detector only detects chromophoric compounds.
The separation in High-performance liquid chromatography has less efficiency than GC.
It is more difficult for the beginner.
HPLC pump process reliability relies on of cleanliness of the sample, mobile phase, and proper operation of the system.

Question 2:

How can you separate a mixture of polar amino acids into its respective components? Justify your answer.

Solution:

We can separate a mixture of polar amino acids into their respective components with the help of ion-exchange chromatography.

Justification:

The amino acids are separated by elution with sodium citrate buffer and they are displaced as a function of their pI values by changing the pH of the buffer solution. The effluent from the column is collected and analyzed for amino acid content.