S.N. | Character | DNA Replication | Transcription |
1. | Definition | DNA Replication is the process of making new copies of DNA | Transcription is the process by which DNA is copied (transcribed) to RNA |
2. | Significance | DNA Replication is important for properly regulating the growth of division of cells | Transcription of DNA is the method for regulating gene expression |
3. | Transfer of Genetic Information | From DNA to DNA | From DNA to RNA |
4. | Occurs during | S phase of cell cycle. | Occurs in the G1 and G2 phases of cell cycle. |
5. | Motive | Occurs in preparation for cell division. | Occurs in preparation for protein translation. |
6. | Involved in | Cell division | Gene expression |
7. | Raw Materials | dATP, dGTP, dTTP and dCTP serve as raw materials. | ATP, UTP, GTP and CTP serve as raw materials. |
8. | Template | Both DNA strands | Single DNA strand |
9. | Primers | Requires RNA primer to start replication. | No primer is required for initiation. |
10. | Enzymes Required | DNA Helicase, DNA Polymerase | Transcriptase (type of DNA Helicase), RNA polymerase |
11. | Unwinding and Splitting | It involves unwinding and splitting of the entire DNA molecule. | It involves unwinding and splitting of only those genes which are to be transcribed. |
12. | Base pairing | Adenine pairs with thymine. | Adenine pairs with uracil instead of thymine. |
13. | Copying of Template | The entire template strand is copied. | Only the portion of the template DNA that codes for required genes is transcribed, or copied. |
14. | Product | Two daughter DNA | mRNA, tRNA, rRNA and noncoding RNA( like microRNA) |
15. | Strands in product | Double stranded DNA | Single stranded RNA |
16. | Postformation | Joining of Okazaki fragments | RNA editing |
17. | Processing | It produces normal DNA molecules that do not need any processing. | It produces primary RNA transcript molecule which needs processing to acquire final form and size. |
18. | Bond | Replicated DNA strand remains hydrogen bonded to its template DNA strand.
| Transcribed RNA strand separates from its DNA template strand |
19. | Migration from the site of formation | Products remain within nucleus. | Greater part of the product passes from nucleus into the cytoplasm. |
20. | Degradation of Product formed | Products are not degraded. | Products are degraded after their function is over. |
21. | Rate of Production | The rate of replication is typically 20 times faster than transcription and six or more replication forks may be present at the same time on the chromosome. | Comparatively slower. |
22. | Process followed by | Transcription/ Next replication | Translation. Although some RNA are the final product themselves. |
23. | Significance | Conserve the entire genome for next generation. | Necessary for protein synthesis. |
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